Comparison of Biochemical, Antimicrobial and Cytotoxic Activities of Different Propolis Samples from Malatya and Bilecik

Merve Keskin,Ercan Karabulut,Eray Tatlici,Semiramis Karlidag,Ozfer Yesilada,Elif Apohan,Ozgur Yilmaz

doi:10.37358/rc.20.5.8144

Abstract

Propolis is a resinous natural mixtures collected and produced by honey bees. It is rich in essential oils and phenolic components so it has high levels of antioxidant, antimicrobial, anti-inflammatory and anti-tumoral activity. In this study the biochemical activity of propolis extracts were determined. The antimicrobial activity and cytotoxic activity of the extracts of the nine different propolis samples were invastigated. Their antimicrobial activities were tested by microdillution metod and define as minimum inhibitory concentration (MIC). Chemical composition of extracts was determined by using GC-MS equipment. Total phenolic content and antioxidant activity of the extracts was measured. Antimicrobial and cytotoxic activity of the extracts was carried out as well. All of the extracts showed antimicrobial activity on bacteria and yeasts used. Extracts had generally lower MIC values on yeasts. Therefore, yeasts were detected as more susceptible against the propolis extracts than the bacteria. Cytotoxic activity of extract were determined aganist A549 and Beas2B cell lines and IC50 values were calculated. Ma-Arapgir had the highest cytotoxic activity on A549 and Beas2B. They were determined as 6.72 and 26.44 mg/mL, respectively. It could be concluded that propolis extracts have antimicrobial and cytotoxic activity thus, propolis could be used in the treatment of cancer.

Highlights

Apitherapy defined as a treatment made with bee products like honey, propolis bee venom etc
Propolis has gained significant importance in such purposes due to its rich biologically active components. It is a natural mixture collected by honeybees from bud exudates of plants
The amount of balsam determined in Bilecik and Malatya propolis ranged between 10.3% and 40.6%

Summary

Introduction

Apitherapy defined as a treatment made with bee products like honey, propolis bee venom etc. They have promising benefits on human health. Propolis has gained significant importance in such purposes due to its rich biologically active components. It is a natural mixture collected by honeybees from bud exudates of plants. Types and amount of both volatile and phenolic constituents vary according to the source of resin. The physical and chemical properties of propolis vary according to obtained geographical region (resin source), it exhibits high antioxidant, antimicrobial, anti-inflammatory and antitumor properties due to its essential oils and polyphenols [1,2,3]

Methods

Results

Conclusion