Abstract
Haemoglobin is one of the commonest tests required in pathology labs. There are many methods of haemoglobin estimation. Common methods used are sahli’s acid hematin method, photometric cyanmethemoglobin method with hemoglobin estimation by colorimeter, automated method by cell counter.To evaluate accuracy of hemoglobin estimation by manual photometric method versus automated method by hematology cell counters using the same sample at the same time.Blood samples of 460 adult patients and 72 children (<15 Yrs), including outdoor and indoor, between May 2019 to Aug 2019, attending H.I.M.S.were collected in EDTA tubes. Samples were properly mixed on blood shaker. Hemoglobin estimation done by photometric cyanmethemoglobin method by AIMIL digital colorimeter and by Mindray (BC5150) automated cell counter.Results by photometric method showed higher mean value compared to automated method by 2.52%. Commercial control results showed 2.2% coefficient of variation by Mindray cell counter and 2.9% by photometerBoth methods are accurate with 2.52% more mean value in photometric method. When Haemoglobin is the only test required, photometric method is cost effective and feasible. If sample size is large as in tertiary care hospitals and multiple parameters like complete blood count (CBC) are needed, automated method is time effective and feasible
Highlights
Hemoglobin comprises of four globin protein subunits, each having one polypeptide chain and one heme group
Control blood was used from Diagon
A total of 532 samples were processed for hemoglobin estimation
Summary
Hemoglobin comprises of four globin protein subunits, each having one polypeptide chain and one heme group. Hemoglobin being one of the commonest investigations in laboratory, we require economical, feasible and accurate method. Common methods used are sahli’s acid hematin method, photometric cyanmethemoglobin method with hemoglobin estimation by colorimeter, automated method by cell counter. Objective: To evaluate accuracy of hemoglobin estimation by manual photometric method versus automated method by hematology cell counters using the same sample at the same time. Hemoglobin estimation done by photometric cyanmethemoglobin method by AIMIL digital colorimeter and by Mindray (BC5150) automated cell counter. When Haemoglobin is the only test required, photometric method is cost effective and feasible. If sample size is large as in tertiary care hospitals and multiple parameters like complete blood count (CBC) are needed, automated method is time effective and feasible
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