Abstract
The variation on aspartate aminotransferase (AAT) isozymes of flower buds of Rhododendron kiusianum, R. kiusianum var. sataense, and R. kaempferi, which are considered to be the parental species of Kurume azalea (R. obtusum), and Kurume azalea cultivars was studied by polyacrylamide gel electrophoresis. AAT isozymes of Kurume azalea were compared with those of three wild evergreen azaleas to seek information on the origin of the domestic cultivars. Three loci (Aat-1, Aat-2, and Aat-3) were identified for AAT. The Aat-2 locus in the three wild evergreen azaleas had two common presumptive alleles (Aat-2100 and Aat-280), and two species-specific presumptive alleles ; Aat-286 for R. kiusianum var. sataense and Aat-290 for R. kaempferi. Aat-3 locus shared the common presumptive alleles (Aat-367, Aat-358, and Aat-349) with three species. Aat-2 locus of Kurume azalea contained four alleles detected in the above three species and also Aat-2112 and Aat-2104 alleles which were not found in R. kiusianum, R. kiusianum var. sataense, and R. kaempferi. These results indicate that Kurume azalea contains genes from R. kiusianum var. sataense and R. kaempferi, and some other related species than the above three wild ones.
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