Abstract
ABSTRACT Powdered dry leaves of green tea were extracted with methanol. Green tea extract was tested for antioxidative activity in comparison with antioxidants (sodium metabisulfite and butylated hydro-xytoluene) at 0.1,0.5,1.0, and 2.0% w/w in 2% w/w hydroquinone cream. The systems were incubated in a dark room at 25°C ± 0.5°C and 45°C ± 0.5°C for 3 months. The physical stability and the percentages of hydroquinone remaining after 2 weeks and 1, 2, and 3 months were determined by UV spectrophotometry at 294 nm according to official standard procedures. The experiment revealed that oxidation degradation of hydroquinone was accelerated by heat even with existence of antioxidants. The green tea extract at 1.0 and 2.0% can be used as a double action (both water- and oil-soluble) antioxidant, having 77 and 81% (at 25°C), and 58 and 62% (at 45°C) hydroquinone remaining, respectively, after 3 months. These results suggested the possibility of using the green tea extract as an effective natural antioxidant for substances that are oxidation-susceptible.
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