Comparison of antibacterial and cytotoxic activities of phytosynthesized ZnONPs by leaves extract of Daphne mucronata at different salt sources

Abstract

ABSTRACT In this research, an aqueous extract of Daphne mucronata leaves with different salt sources including Zn(NO3)2 and ZnSO4 solutions were used to synthesize ZnO-N and ZnO-S NPs, respectively. The physicochemical, antibacterial, antioxidant, and cytotoxic properties of these NPs were also surveyed. SEM and TEM images confirmed that the ZnO-N and ZnO-S NPs were polydispersed with spherical and plate shape and average diameters of 129 ± 45.49 nm and 87 ± 31.61 nm, respectively. The minimum bactericidal concentration (MBC) values for both NPs against bacterial strains were 2 mg/ml. The biofilm growth in biofilm producer strains was completely inhibited at minimum inhibitory concentration (MIC). The IC50 values for ZnO-N and ZnO-S NPs were calculated as 1.946 mg/ml and 0.369 mg/ml, respectively. Finally, due to the antioxidant, antimicrobial, and cytotoxic properties of zinc oxide NPs, green synthesis of ZnONPs using D. mucronata can improve their applications in a wide range of clinical areas.