Abstract
AbstractSeparation of anionic, cationic, and amphoteric surfactants containing n‐dodecyl groups and hydrophilic moieties was done by high‐performance liquid chromatography (HPLC) and high‐performance capillary electrophoresis (HPCE), and an ultraviolet‐visible detector. Quantitation of surfactants in commercial cosmetic and toiletry products was also done using similar methods. Conditions used to separate mixtures of surfactants by HPLC are as follows: stationary phase: ODS 2; mobile phase: MeOH/H2O (80∶20, vol/vol) containing 1.0M NH4Cl, 0.003 M tetrabutylammonium hydrogen sulfate, and 0.005 M diammonium phosphate buffer solution at pH=6.0. Conditions for HPCE are as follows: an uncoated capillary (100 μm i.d., 110 cm in length, effective length of 75 cm) with 25 kV of applied voltage, and an aqueous buffer containing 80 mM sodium borate/20 mM NaOH at pH=9.2. Surfactants were eluted within 15 min. The accuracy of both techniques was evaluated by analyzing the recovery ratio of surfactants in the mixture.
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