Abstract
BackgroundToxoplasma gondii is an opportunistic protozoan parasite that can infect all warm-blooded animals including humans and cause serious clinical manifestations. Toxoplasmosis can be diagnosed using histological, serological, and molecular methods. In this study, we aimed to detect T. gondii RE gene in various human samples by in house and commercial real time polymerase chain reactions.MethodsA total of 38 suspected cases of toxoplasmosis [peripheral blood (n:12), amnion fluid (n:11), tissue (n:9), cerebrospinal fluid (n:5), and intraocular fluid (n:1)] were included to the study. An in house and a commercial RT-PCR were applied to investigate the T. gondii RE gene in these samples.ResultsThe compatibility rate of the two tests was 94.7% (37/38). When the commercial RT-PCR kit was taken as reference, the sensitivity and specificity of in house RT-PCR test was 87.5 and 100%. When the in house RT-PCR test was taken as reference, the commercial RT-PCR kit has 100% sensitivity and 96.8% specificity. Incompatibility was detected in only in a buffy coat sample with high protein content.ConclusionsBoth the commercial and in house RT-PCR tests can be used to investigate T. gondii RE gene in various clinical specimens with their high sensitivity and specificity. In house RT-PCR assay can be favorable due to cost savings compared to using the commercial test.
Highlights
Toxoplasma gondii is an opportunistic protozoan parasite that can infect all warm-blooded animals including humans and cause serious clinical manifestations
In order to rule out this suspicion, amniocentesis is recommended during the 16-18th weeks of gestation and amniotic fluid is investigated with Polymerase chain reaction (PCR) to detect T. gondii Deoxyribonucleic acid (DNA) [1, 7]
According to the results, among the 38 clinical samples analyzed, T. gondii Repeat element (RE) gene was not detected in 30 of them and T. gondii RE gene was detected in 6 samples by both in house Real Time PCR and commercial Real Time PCR (Table 1)
Summary
Toxoplasma gondii is an opportunistic protozoan parasite that can infect all warm-blooded animals including humans and cause serious clinical manifestations. Toxoplasma gondii is an opportunistic protozoan parasite that can infect all warm-blooded animals including birds and can cause serious clinical manifestations. Toxoplasmosis can be diagnosed using histological, serological, and molecular methods [1, 3,4,5,6]. Serological and molecular diagnostic methods are frequently used. When IgM positivity and low IgG avidity are detected in the pregnant woman, there is a risk of congenital toxoplasmosis in the fetus [1]. Molecular techniques become important due to Döşkaya et al BMC Infectious Diseases (2019) 19:1042 decreased levels of antibodies in immune suppressed patients [8,9,10]
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