Abstract
The AFLP and SSR markers were used to estimate the genetic diversity of 25 Brassica napus hybrids attending Guizhou regional test. The fingerprints obtained with both the AFLP and SSR markers revealed high levels of heterozygosity. Nine AFLP primer combinations produced 16 loci, while 11 SSR primer pairs generated 22 loci. The mean of expected heterozygosity, Shannon’s information index, and genetic differentiation obtained by SSR were higher than those by AFLP, indicating that the SSR methodology evaluated genetic diversity among B. napus more efficiently than the AFLP approach. The higher level of genetic diversity detected by SSR markers was contributed to the lower genetic similarity estimates based on SSR markers (mean 0.69) as compared to AFLP markers (mean 0.73). While the AFLP technique was suitable for identification and DNA fingerprinting of B. napus germplasm. Based on AFLP and SSR analysis, it was concluded that B. napus hybrids had high level genetic diversity.
Highlights
1.1 Brassica napus of Guizhou Brassica napus is one of the most important sources of vegetable oil in Guizhou, China, and is the second most www.ccsenet.org/jas important rapeseed crop in the world after soybean
Previous reports revealed that the level of polymorphism for B. napus is less than 45% (Cheung et al, 1997; Kresovich et al, 1995; Uzunova et al, 1995), whereas in B. oleracea it can be higher than 80% (Cheung et al, 1997), and in B. juncea a polymorphism of approximately 60% (Cheung et al, 1997)
The polymorphism revealed by the amplified fragment length polymorphic (AFLP) and simple sequence repeat (SSR) markers is lower than 45%, which supported the previous reports
Summary
1.1 Brassica napus of Guizhou Brassica napus is one of the most important sources of vegetable oil in Guizhou, China, and is the second most www.ccsenet.org/jas important rapeseed crop in the world after soybean. In 2006, rapeseed cultivation area in Guizhou was about 1.27 million acre, of which B. napus, B. juncea and B. campestris make up 70% - 75%, 10%, and 15%, respectively (Rao et al, 2005). During long term natural and artificial selection, a large number of B. napus hybrids were developed. With the application and dissemination of elite hybrid rapeseed, yield of B. napus has been improved and new cultivars are introduced to update the assortment. Regional tests provide useful information for the registration and protection of B. napus hybrids. The genetic diversity of B. napus hybrids attending Guizhou regional test remains unevaluated, and the genotypes selected and exploited for extensive planting are very much limited
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