Abstract
A comparative study of various adsorption-elution methods for concentration of viruses seeded into 51. tap water was carried out. The methods were: 1. (1) Aluminum hydroxide precipitation; 2. (2) Adsorption to cellulose nitrate membrane filter and elution with glycine buffer at pH 11.5; 3. (3) Insoluble polyelectrolytes (PE 60); 4. (4) Adsorption to cellulose nitrate membrane filter and elution with 3% beef extract. The aluminum hydroxide precipitation method served as the standard procedure for comparison with our previously published methods. The various methods were compared, using attenuated poliovirus I and enteroviruses recovered from sewage. The results show that the membrane filter elution with 3% beef extract and the PE 60 method provide a good average recovery of 70% and above for both seeded poliovirus and diluted sewage. On the other hand, the membrane filter of elution with glycine buffer at pH 11.5, provides an average recovery of 65–67%.
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