Abstract
Objectives: The aim of this study was to assess whether there are differences between the clustering of isolates collected between 2003 and 2005 from two different hospitals in Tunisia and France. Methods: A selection of 62 isolates of A. baumannii was studied; 31 from the French Hospital (Poitiers) and 31 from Tunisian Hospital (Rabta). Antibiograms were done using the disc diffusion method. The presence of integrons class 1 and 2 was studied by PCR. Molecular relationship was studied by Random Amplified Polymorphic DNA (RAPD) and Pulsed Field Gel Electrophoresis (PFGE). The sequence typing of adeB gene was determined to identify intraspecific groups. Results: The present study successfully focuses to compare epidemiologic status between two hospitals study. PFGE and RAPD methods were useful to distinguish epidemic and endemic clones in the two collections study. Sequence analysis of an 850-bp internal fragment drug efflux gene adeB revealed 9 novel sequence types (STs). Conclusion: We, statistically, found no significant difference related to the epidemiological situation. This study showed that different genetic types of A.baumannii were found in the two collection strains. Epidemics ones were essentially confined in the ICU and were persisting during the two years study. However, more control procedures had to be used on these clones for the Tunisian hospital.
Highlights
A.baumannii is a Gram negative coccobacillus, largely confined in the nosocomial environment that emerged as an important nosocomial pathogen in recent years
The results show that the levels had achieved the same degree of resistance except for imipenem which was two times higher for the Tunisian isolates
Tunisian isolates were scattered in 12 clusters by the same classification representing different combinations of antibiotics resistance with 5 clusters containing only one isolate
Summary
A.baumannii is a Gram negative coccobacillus, largely confined in the nosocomial environment that emerged as an important nosocomial pathogen in recent years. Hospital outbreaks caused by this organism have increased worldwide [1,2,3,4]. It was demonstrated that A. baumannii multiresistant strains were essentially isolated in intensive care units (ICU) in critically ill persons [5,6,7]. The aim of this study was to compare the clustering relationship of A. baumannii isolates between two different hospitals, located in Tunisia and France. This study was not intended to be a formal assessment of the epidemiological aspects of this pathogen
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
