Abstract
A resonant mirror biosensor, IAsys, and a quartz crystal microbalance (QCM) are known independently as surface sensitive analytical devices capable of label-free and in situ bioassays. In this study, an IAsys and a QCM are employed for a new study on the action mechanism of Paeoniae Radix 801 (P. radix 801) by detecting the specific interaction between P. radix 801 and endothelin-1 (ET-1). In the experiments, ET-1 was immobilized on the surfaces of the IAsys cuvette and the QCM substrate by surface modification techniques, and then P. radix 801 solution was contacted to the cuvette and the substrate, separately. Then, the binding and interaction process between P. radix 801 and ET-1 was monitored by IAsys and QCM, respectively. The experimental results showed that P. radix 801 binds ET-1 specifically. The IAsys and QCM response curves to the ET-1 immobilization and P. radix 801 binding are similar in reaction process, but different in binding profiles, reflecting different resonation principles. Although both IAsys and QCM could detect the interaction of P. radix 801 and ET-1 with high reproducibility and reliability through optimization of the ET-1 coating, the reproducibility and reliability obtained by IAsys are better than those obtained by QCM, since the QCM frequency is more sensitive to temperature fluctuations, atmospheric changes and mechanical disturbances. However, IAsys and QCM are generally potent and reliable tools to study the interaction of P. radix 801 and ET-1, and can conclusively be applied to the action mechanism of P. radix 801.
Highlights
Resonant mirror biosensor (IAsys) and quartz crystal microbalance (QCM) have been known independently as surface sensitive analytical devices capable of on-line monitoring of interfacial reactions and thin film depositions [1,2,3]
As mentioned in Experimental Section, the ET-1 molecules were immobilized on the surface of the IAsys cuvette by the ester exchange reaction of NHS to the COOH groups of CMD
The preliminary results showed that the average immobilized amount of ET-1 in IAsys is 1.097 ng mm-2, on the other hand, that in QCM is 278.880 ng cm-2 (2.79 ng mm-2), in spite of the almost same interaction amounts of P. radix
Summary
Resonant mirror biosensor (IAsys) and quartz crystal microbalance (QCM) have been known independently as surface sensitive analytical devices capable of on-line monitoring of interfacial reactions and thin film depositions [1,2,3]. Combined IAsys and QCM data collection and analysis allow one to take the advantage of the features, to test the validity of the assumptions and to gain a better understanding of a specific interfacial reaction. Both the devices have been widely used for biological analysis, clinical diagnosis, and environmental monitoring [12,13]. Paeoniae Radix 801 (P. radix 801), a component of Radix Paeonia Rubra, is mainly composed of propyl gallate It is one of the active ingredients of the blood-activating and stasis-eliminating traditional Chinese medicine (TCM) [20]. Comparison of the two devices with respect to reproducibility and reliability are conducted
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