Abstract

Insecticide toxicity is commonly evaluated for disease vectors by either the WHO test or Bottle assay. More recently, a high-throughput screening (HTS) system was developed for testing insecticide effects on mosquito behavior and mortality. We compared HTS with the Bottle assay to evaluate the toxicity of insecticides in a population of Aedes aegypti from Thailand. Both the HTS and Bottle assay system were determined to be equivalent. The two systems mainly differed (1) in reaction time, with mosquitoes reacting faster in the Bottle assay than HTS, (2) in knockdown and mortality at low doses. This information will guide the testing protocol for evaluating chemical effects on behavioral responses in various vector populations.©Pesticide Science Society of Japan.

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