Abstract
Colony hybridizations with a gene probe for enumeration of 2,4-dichlorophenoxy-acetic acid (2,4-D)-degrading bacteria were compared with classical enrichment and radiolabel most-probable-number (MPN) assay methods. Two natural water samples (rivers) and raw sewage were tested by each method. UV scans of enrichment cultures revealed 2,4-D degradation with raw sewage occurred in 4–11 days, 4–>22 days with Mary's River water, and 5–>22 days with Willamette River water. [14C]-2,4-D MPN analysis, measuring release of14CO2, yielded estimates of bacteria per milliliter able to degrade 2,4-D. Raw sewage estimates were 1.4 × 105 2,4-D degraders/ml, Mary's River >1.6 × 105/ml, and Willamette River water 1.6 × 104/ml. Activities noted by UV scan enrichment data supported these results.
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