Abstract
The use of scanned electron probes, rather than the wide-beam illumination of standard transmission electron microscopy, allows the structural biologist to image 3-D cellular and tissue ultrastructure by taking full advantage of the physical interactions between the incoming electrons and the specimen. For example, in serial block face scanning electron microscopy (SBF-SEM), a low-energy (∼1 keV electron probe) produces a backscattered electron signal originating from a thin approximately 25-nm layer below the face of a heavy-atom stained, resin-embedded block.
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