Abstract
Aromatic rice lines were examined for 2-Acetyl-1-Pyrroline (2AP) content in leaf tissue at five different growth stages (tillering, panicle initiation, 50% heading, booting, and maturity). A small plot trial with plot size of 1.42 m × 4.88 m (7 row-plots) was arranged in completely randomize design with three replications. Dry-seeded, delayed flood cultural practice was used in this study. The experiment was conducted at three locations. The average 2AP concentrations in leaf tissue at tillering stages were higher than the other four growth stages. 2AP levels were declined when rice plant reached booting. AP levels decreased slightly at heading stage and decreased significantly at maturity. There was no significant different between 2AP in leaf at 50% heading from three locations as well as the 2AP content in rice grain. Correlations between 2AP in leaf and 2AP in grain were significantly in all five growth stages. The highest correlation coefficient was found between 2AP in leaf at booting and grain (r = 0.811**) and lowest was in the leaf at harvest (r = 0.564**). Results indicated that 2AP could be determined in leaf tissue at early growth stage.
Highlights
IntroductionThe aroma quality of aromatic rice in sensory evaluations showed a strong correlation to 2AP concentration, which is formed in aerial parts of plants during growth in paddy fields
Aromatic rice varieties are very popular in South and Southeast Asia and have recently gained wider acceptance in the USA and Europe
Results indicated that 2AP could be determined in leaf tissue at early growth stage
Summary
The aroma quality of aromatic rice in sensory evaluations showed a strong correlation to 2AP concentration, which is formed in aerial parts of plants during growth in paddy fields. There are several chemical compounds that control the aroma and flavor. Buttery et al [1] reported that 2AP was a key aroma compound of cooked rice. Direct analysis method using cooked rice has been the major 2AP analysis used by many researchers [3] [7] [8]. A number of researchers have reported the analysis of 2AP directly from uncooked rice [9] [10] [11]
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