Comparison Distribution of Vibrio Species in Stocking to Harvesting Process of Shrimp at Commercialize Shrimp Farm

Abstract

Vibrio species is one of the pathogenic bacteria infecting shrimps in aquaculture farm has caused severe loss to the aquaculture farm. The aim of this research is to isolate and compare the presence of Vibrio spp. in commercialize shrimp farm during the stocking process and the final harvest phase of shrimp productions. Quantification and isolation were conducted and measured using Log CFU (colony forming units) counts and morphological investigation gram staining and conventional biochemical test identification. A total of sixty-four (n=64) sediment and water samples were collected from the inlet and outlet of the two selected shrimp ponds (Pond A and Pond B) in Kampung Telaga Air, Kuching, Sarawak. As a result, the bacteria count from water samples during stocking period at both shrimps ponnds were 6.6 Log CFU/ml and 5.6 Log CFU/ml while during harvesting period were 5.3 Log CFU/ml and 5.8 Log CFU/ml. Meanwhile, bacteria count sediments samples during stocking period at both shrimp ponds were 5.5 Log CFU/g and 5.7 Log CFU/g whereas harvesting period at showed both shrimps pond were 5.2 Log CFU/g and 4.8 Log CFU/g. The finding showed the Vibrio sp. identified in sediment samples were V. parahemolyticus with 27%, followed by V. alginolyticus (22%), V. cholerae (16%). V. fluvialis (14%) and 7% of V. fulmisi and V. vulnificus and V. mimicus. Meanwhile, the Vibrio sp. identified in water samples were V. fluvialis with 29%, followed by V. alginolyticus (22%), V. parahemolyticus (19%) and 10 % were V. fulmisi , V. mimicus and V. vulnificus. The study has been carried out further by investigated the Antibiotic Susceptibility Test (AST) using erythromycin (15 ug/ml), ampicillin (10 ug/ml), and streptomycin (25 ug/ml). The positive cultures from sediment samples showed Ampicillin resistant profile was > 0.2 MAR index while fifty percent (50%) of the isolates from water samples were resistant to ampicillin. Therefore, this study has profound implications for further monitoring process from the stocking process towards the harvesting process in order to prevent any Vibrio sp. infection which caused shrimp’s disease or food safety issue related to public consumption or of shrimps in future.