Abstract
The emergence of outbreaks of foodborne illness is closely associated with food contamination caused by various enteric pathogens, such as Escherichia coli O157:H7, Listeria monocytogenes, Salmonella enterica serovar Enteritidis, and Staphylococcus aureus. The control of enteric pathogens poses a challenge due to the fact that these pathogens can persist for a long period of time in the environment. The rapid detection of pathogenic organisms plays a crucial role in the prevention and identification of crises related to health, safety, and well-being. Improper sample handling and processing may influence the diagnostic efficacy and accuracy. The aim of the present study was to compare the preservation capacity for enteric bacteria between Whatman Flinders Technology Associates (FTA) cards and swabs for reverse transcription-quantitative PCR (RT-qPCR) detection. It was found that Whatman FTA cards exhibited an improved preservation capacity for five types (both laboratory and environmental strains) of enteric bacteria, including Escherichia coli O157:H7, Listeria monocytogenes, Salmonella enterica serovar Enteritidis, and Staphylococcus aureus for RT-qPCR detection. Hence, Whatman FTA cards may be a suitable tool for the routine isolation of foodborne bacteria for molecular diagnosis. Therefore, the use of Whatman FTA cards for sample collection and preservation may increase sensitivity and accuracy for bacteria isolation and diagnosis.
Highlights
E emergence of outbreaks of foodborne illness is closely related to food contamination caused by various enteric pathogens, which has been reported to cause high morbidity and mortality among humans worldwide [2]. e World Health Organization (WHO) reported that there were approximately a few million cases of foodborne illness caused by pathogenic bacteria each year [3]. e control of enteric pathogens poses a challenge, as these pathogens can persist for a long period of time in the environment [2]
TM compared the differences between Whatman Flinders Technology Associates (FTA) Elute cards and conventional swabs for the handling of enteric bacteria, including Escherichia coli O157:H7, Listeria monocytogenes, Salmonella enterica serovar Enteritidis, and Staphylococcus aureus when detected by reverse transcription-quantitative PCR (RT-qPCR) assay. e information presented may be helpful for selecting the optimal handling and process method for bacterial diagnosis
It was found that the CT values of the stx1 gene of Escherichia coli O157:H7 on the Whatman Elute FTA card were significantly lower than those on the conventional swab (Figures 2(a) and 2(b), n 6; P < 0.05, P < 0.01, and P < 0.001)
Summary
E emergence of outbreaks of foodborne illness is closely related to food contamination caused by various enteric pathogens, which has been reported to cause high morbidity and mortality among humans worldwide [2]. e World Health Organization (WHO) reported that there were approximately a few million cases of foodborne illness caused by pathogenic bacteria each year [3]. e control of enteric pathogens poses a challenge, as these pathogens can persist for a long period of time in the environment [2]. TM compared the differences between Whatman FTA Elute cards and conventional swabs for the handling of enteric bacteria, including Escherichia coli O157:H7, Listeria monocytogenes, Salmonella enterica serovar Enteritidis, and Staphylococcus aureus when detected by reverse transcription-quantitative PCR (RT-qPCR) assay.
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Schedule a callMore From: The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale
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