Abstract
The enzymatic synthesis of homogeneous tri-docosahexaenoylglycerol from glycerol and ethyl docosahexaenoate in a solvent-free medium was achieved using Novozym SP 435 (immobilized lipase from Candida antarctica). Two processes were tested: a constant stirred tank reactor with N2 bubbling through and a constant stirred tank reactor under vacuum. The first experimental apparatus was clearly found to be better than the other, giving 100% (w/w) conversion after 10 h.
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