Abstract
An investigation was carried out to study the effects of single and double injection of given dose of pituitary gland (PG) on the breeding performance viz., ovulation response, fertilization, and hatching of climbing perch, Anabas testudineus was tested. Pituitary gland dose of 1.2 mg/100g was used in this experiment. Twenty female fish was arranged into two treatments: T1 and T2, each with 10 replications. In T1, the entire dose was administered in a single injection and in T2 the same dose was given in two equal injections three hr apart. The experiment was laid in completely randomized design (CRD). It was found that administration of PG in split doses (double injection) had no additional advantages (ovulation 100%, fertilization 81.56% and hatching 72.68%) over the single injection (ovulation 100%, fertilization 83.03% and hatching 75.48%) treatment. Similarly, the ovulation time of the fish did not vary significantly in either single or double injection of PG (single injection 7.12 hr; double injection 7.20 hr). The results demonstrated that splitting of pituitary gland dose (double injection) has no additional advantages on breeding performance of A. testudineus. Therefore, single injection may be preferred over the second injection for breeding of this fish. 
 
 Key words: Breeding, pituitary gland (PG), fertilization, hatching 
 
 J. bio-sci. 14: 57-60, 2006
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