Abstract
This paper deals with the specificity of the anti 3′,5′-cyclic AMP antibodies which can be obtained with 2′- O-succinyl cyclic AMP-albumin as an immunogen. The binding of the hapten and its analogs was measured by equilibrium dialysis. Rat and rabbit antibodies were compared. In both cases the best ligands for the anti-hapten antibodies are 2′- O-acylated derivatives of cyclic AMP: the dissociation constants are below 10 −10 m. Cyclic AMP itself and its 6 N, 2′- O-diacylated derivatives are recognized less efficiently; their dissociation constants lie around 10 −8 m, similar to that of natural cyclic AMP binding proteins. Other nucleotides lacking either adenine or the 3′,5′-phosphate ring are not recognized. Three different populations of antibodies were detected by a more detailed analysis of the equilibrium curves.
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