Abstract
Mutagenesis is an important tool for breeding and genomic research. In this study, the germinated seeds and isolated microspores of a double haploid line ‘FT’ were treated with EMS, respectively, with the aim of comparing the effects of the two approaches on generating mutants in Chinese cabbage. For microspore EMS mutagenesis, the isolated microspores were treated with 0.12% EMS for 20 min, a total of 1268 plantlets were obtained, and 15 M1 mutants were screened with a mutation frequency of 1.2%. For seed EMS mutagenesis, 7800 germinated seeds were treated with 0.8% EMS for 12 h, and a total of 701 M2 mutants were screened, with a mutation frequency of 18.78%. In total, 716 mutants with heritable morphological variation including leaf color, leaf shape, leafy head, bolting, and fertility, were obtained from the EMS mutagenesis experiments. Homozygous mutant plants could be screened from M1 lines by microspore mutagenesis, and M2 lines by seed mutagenesis. The mutation frequency was higher in seed mutagenesis than in microspore mutagenesis. Based on these results, we propose that seed EMS mutagenesis is more suitable to generate a large-scale mutant library, and the microspore EMS mutagenesis is conducive to rapidly obtaining homozygous mutants.
Highlights
Accepted: 4 March 2022Plant mutants are ideal materials for discovering new genes and revealing their functions, which are widely used in functional genomics researches [1–6]
Mutants are important for the investigation of gene function in crop plants
Wang et obtained zebra-15 mutants from the restorer line Jinhui10 (Oryza sativa L. ssp. indica) al. [18] obtained zebra-15 mutants from the restorer line Jinhui10 (Oryza sativa L. ssp. indica) by treatment with Ethyl methanesulfonate (EMS) for studies of chlorophyll
Summary
Accepted: 4 March 2022Plant mutants are ideal materials for discovering new genes and revealing their functions, which are widely used in functional genomics researches [1–6]. Plant mutant libraries are typically constructed by artificial mutagenesis, including chemical mutagenesis [7,8], physical mutagenesis [9,10], and insertion mutagenesis [11–13]. Ethyl methanesulfonate (EMS) is a widely used chemical mutagenic agent [14,15]. EMS mutagenesis has been applied to construct the mutant libraries in a wide range of crops, such as rice [17,18], Arabidopsis thaliana [7,19], wheat [20–23], tomato [24,25], soybean [26–29], maize [30], peanut [31], and Brassica napus [32,33]. Lu et al [34] treated an inbred line ‘A03’ seeds with 0.4% EMS for 16 h and constructed a mutant library, containing 4253 M1 families. Lu et al [35] mutagenized the buds with different concentrations of EMS solution, and the isolated microspore culture was conducted where a total of 142 mutants were identified. Huang et al [36] treated the microspores of a Published: 8 March 2022
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