Comparison between enzyme-linked immuno-sorbent assay and multiplex assay for serological markers of Celiac Disease detection

Abstract

Event Abstract Back to Event Comparison between enzyme-linked immuno-sorbent assay and multiplex assay for serological markers of Celiac Disease detection Lydia Martín-Martín1*, Laura Herráiz-Nicuesa1, Yaciris Oleo-Pérez1, Silvia Sánchez-Ramón2 and Margarita Rodriguez-Mahou1 1 Hospital General Universitario Gregorio Marañón, Spain 2 Hospital Clínico San Carlos, Spain Objective: To compare ELISA with multiplex assay for anti-deamidated gliadin peptide (anti-DGP) IgG and anti-tissue transglutaminase (anti-tTG) IgA antibodies detection in order to identify the best according to sensivity and specificity. Methods: 101 patients with suspicion or diagnostic of celiac disease (CD) were selected. Each serum was tested by ELISA (Orgentec Diagnostika) and multiplex assay (Bioplex 2200, Bio-Rad) for the anti-DGP IgG and anti-tTG IgA autoantibodies quantification. The association with clinical features was examinated by systematical review of the clinical histories. Also previous CD markers determinations, biopsy and HLA features were studied. Results: Compared to the clinical, biopsy and HLA features, the ELISA showed a sensitivity of 96.15% and a specificity of 38.09 (Positive predictive value (PPV): 79.36%; Negative predictive value (NPV): 80%), whereas multiplex assay had a sensitivity of 79.59% and a specificity of 100% (PPV: 100%; NPV: 68.75%) for anti-DGP IgG antibodies detection. Furthermore, the ELISA showed a sensitivity of 68.42% and a specificity of 100% (PPV: 100%; NPV: 93.18%), and multiplex assay had a sensitivity of 100% and a specificity of 96.34% (PPV: 86.36%; NPV: 100%) for anti-tTG IgA antibodies testing. Conclusions: Taking into consideration the previous CD markers determination and clinical, biopsy and HLA features, ELISA showed a higher sensitivity for anti-DGP IgG antibodies, but specificity was better for multiplex assay. In addition, ELISA showed a higher specificity and multiplex had a better sensitivity for anti-tTG IgA antibodies detection. Keywords: Celiac Disease, ELISA, Multiplex assay, Anti-deamidated gliadin peptide IgG antibodies, Anti-tissue transglutaminase IgA antibodies Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Martín-Martín L, Herráiz-Nicuesa L, Oleo-Pérez Y, Sánchez-Ramón S and Rodriguez-Mahou M (2013). Comparison between enzyme-linked immuno-sorbent assay and multiplex assay for serological markers of Celiac Disease detection. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.01057 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 10 Jul 2013; Published Online: 22 Aug 2013. * Correspondence: Miss. Lydia Martín-Martín, Hospital General Universitario Gregorio Marañón, Madrid, Spain, lydia.martin@salud.madrid.org Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Lydia Martín-Martín Laura Herráiz-Nicuesa Yaciris Oleo-Pérez Silvia Sánchez-Ramón Margarita Rodriguez-Mahou Google Lydia Martín-Martín Laura Herráiz-Nicuesa Yaciris Oleo-Pérez Silvia Sánchez-Ramón Margarita Rodriguez-Mahou Google Scholar Lydia Martín-Martín Laura Herráiz-Nicuesa Yaciris Oleo-Pérez Silvia Sánchez-Ramón Margarita Rodriguez-Mahou PubMed Lydia Martín-Martín Laura Herráiz-Nicuesa Yaciris Oleo-Pérez Silvia Sánchez-Ramón Margarita Rodriguez-Mahou Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.