Abstract
Purpose: To evaluate and compare the promising applications of dextran-coated superparamagnetic iron oxide nanoparticle (dSPION) and magnetoliposome on corneal endothelial cells transplant therapy. Methods: Two kinds of novel superparamagnetic iron oxide nanoparticles (SPIONs), dSPION and magnetoliposome were synthesized, characterized and incorporated to rabbit corneal endothelial cells (RCECs). Uptakes of SPIONs were identified by Prussian blue staining. Cells migrating rates in magnetic field were calculated. Cytotoxicities of the two SPIONs were detected using MTT assay. Result: Magrating rates of dSPION (4 ~ 64 μg/mL) labled RCECs were 24.1±2.4% ~ 78.3±2.2%, and 5.0±1.7% of unlabeled RCECs. (p0.05) Conclusion: RCECs labeled with each SPION migrated significantly in external magnetic field, while cytotoxicities did not revealed. Our study demonstrated the potential applications of dSPION and magnetoliposome on labeling RCECs for cell transplantation.
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