Comparison between Ca2+ and Mg2+ on surface-located ATPase of intact normal and neoplastic human cells in culture.

Abstract

AbstractA Mg2+‐ and Ca2+‐dependent ATPase at the outer surface of human peripheral lymphocytes and hematopoietic cell lines has been studied. The enzyme activity varied about 60 fold between the extremes of the cell lines tested. There was no simple relationship between surface‐ATPase activity and cell surface area. Nor was the presence of villi or any other morphological characteristic at the cell surface decisive for the degree of surface‐ATPase activity. A correlation was, however, found between the ATPase activity and a phenomenon probably involving a contractile process namely immunoglohulin secretion, but it was not possible to establish an interdependence of these cell characteristics.