Comparison between anal cytology, high-resolution anoscopy and HPV DNA genotyping by polymerase chain reaction in the post-treatment follow-up of condylomata acuminata.

Luis Roberto Nadal,Thiago Da Silveira Manzione,Sidney Roberto Nadal,Sarhan Sydney Saad,Gaspar Jesus Lopes Filho,Carmen Ruth Manzione,Helena Passarelli Giroud Joaquim

doi:10.1590/0100-6991e-20202543

Abstract

to evaluate the presence of subclinical HPV-induced anal lesions with anal cytology, High-Resolution Anoscopy (HRA) and HPV genotyping by polymerase chain reaction (PCR) in the follow-up of treated condylomata acuminata (CA). seventy-nine male patients were included. One month after anal CA eradication, the patients underwent brush samples collection for anal cytology and PCR, and HRA with biopsy of acetowhite lesions. These methods were compared within all patients and between groups, according to Human Immunodeficiency Virus (HIV) infection status: HIV-negative; HIV-positive with TCD4 count above and below 350 cells/mm3. the most frequent HPV types were 6 and 16. HPV DNA was isolated in 92%. HIV infection was associated with a higher number of oncogenic HPV types (p=0.038). All patients with negative PCR had negative HRA and cytology. There were no differences in cytological, HRA or histopathological findings between groups. the association of the findings of cytopathology, HRA and genotyping of HPV refined the diagnosis of HPV-induced lesions. The degree of immunodeficiency was not associated with increase in remnant HPV-induced anal lesions.

Highlights

Human Papillomavirus (HPV) is the most common agent of sexually transmitted infections(STI)[1]. It is responsible for anorectal lesions such as condylomata acuminata (CA), squamous cell carcinoma (SCC) and its precursor, intraepithelial neoplasia[1,2]
Our aim is to evaluate whether the association of anal cytopathology, High-Resolution Anoscopy (HRA) and HPV DNA genotyping increases the sensitivity of diagnosis of the remnant HPV-induced subclinical lesions in MSM, as well as to verify if polymerase chain reaction (PCR) genotyping has similar findings to the association between HRA and cytological examinations
The present research aimed to compare HPV genotyping by PCR to HRA, HRA-guided biopsy and cytology after one month of eradication of marginal and anal canal condylomata acuminata

Summary

Introduction

Human Papillomavirus (HPV) is the most common agent of sexually transmitted infections(STI)[1]. It is responsible for anorectal lesions such as condylomata acuminata (CA), squamous cell carcinoma (SCC) and its precursor, intraepithelial neoplasia[1,2]. CA is usually the first clinical manifestation of the STI, and it triggers the workup to diagnose and treat concurrent infections such as the one by the Human Immunodeficiency Virus (HIV)

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