Comparison between Allele Frequencies of Several Strs Loci in Najaf City of Iraq and Middle Province in Iraqi Population

Abstract

The population genetic data and forensic parameters of 15-chromosome short tandem repeat (STR) loci(D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820, TH01, TPOX, CSF1PO, D19S433, D2S1338, D16S539) in Najaf city of Iraq are presented. These loci were detected in a sample of 108 (67 males and 41 females) unrelated healthy individuals. We observed alleles at 15-STR loci using the AmpFlSTR1® Identifiler™ PCR Amplification Kit from Applied Biosystems, The allele frequency spectrum detected in the sample were compared to allele frequencies from three Iraq provinces (Diyala, wasit, and Anbar). Significant global differences in allele frequencies were detected. In our study can be Observed heterozygosity (HO) genotype frequencies and expected heterozygosity (HE) was detected in all 15 markers according to Hardy-Weinberg equilibrium (HWE) the highest heterozygosity is observed for D21S1338 (0.86441) as well as the smallest heterozygosity value is obtained for TPOX (65.00%) These allele frequency databases are suitable for the purpose of identification in paternity or forensic investigations. The combined Matching Probability (CMP) using the 15 STR genetic loci in Iraqi population was estimated at 1 in 8.62157–18 and the Combined Discrimination Power (CDP) was over than 0.9999999, Combined Exclusion Probability (CEP) was 0.999994 which should be sufficient for the identification of any individual. In the end we noticed an 7 locus are non-significant when compared with Hardy-Weinberg (P > 0.05) and the locus are D8S1179, D21S11, D7S820, CSF1PO, D13S317, D16S539, and TPOX.