Abstract

A slot-blot hybridization assay for quantifying the amount of Gaeumannomyces graminis var. tritici (Ggt) in diseased roots and in soil has been developed, using a specific and sensitive DNA probe (pG158). The procedure involves separation of organic matter from soil, DNA extraction of soil organic matter, and a slot-blot hybridization assay. Soil of different types was collected to a depth of 10 cm and organic matter between 0.35–1.4 mm in size was separated from this soil. Using a slot-blot hybridization assay, it was found that Ggt was mostly present in the organic matter fraction greater than 0.5 mm and in soil at a depth of 5 cm.

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