Comparison anti-oxidant and neuroprotective effects of extra-virgin olive oil, donepezil and rosmarinic acid on aluminum chloride-induced Alzheimer’s in rat models

Abstract

In this study, it was aimed to investigate the effects of EVOO, rosmarinic acid and donepezil in Alzheimer's model to be created with AlCl3 in rats. For this reason, administration of 100 mg/kg aluminum chloride (AlC3) for 15 days to Sprague Dawley adult male rats; donepezil, Extra-virgin olive oil (EVOO) and rosmarinic acid were administered to three different groups for 21 days by applying treatment protocols. With this study, we were able to demonstrate that cognitive impairment has been occurred after 15 days of AlCl3 administration by oral gavage and treatment protocols prevented the occurrence of AD pathology histopathologically. We also showed that oxidative damage findings which are positively Congo-red stained cell cytoplasm and impaired cell integrity have been observed in serum and hippocampus. Besides, treatment groups showed better cognitive and motor performance, and there was no damage to the cells in control and treatment groups. In the rotarod motor performance test, a significant increase was observed in the donepezil group compared to the AlCl3 group at speeds of 26 and 30 rpm. In MVM, on the 5th day of the experiment, a significant increase was observed in the donepezil group compared to the AlCl3 group, as they were spending more time in the hidden platform area. These results show that 15 days of aluminum exposure is effective in creating a moderate Alzheimer’s pathology, but further chronic research is necessary to explain the efficiencies of rosmarinic acid and EVOO in treatment.