Abstract
The monitoring outbreaks of bark beetles, flight activity of imago and development of larvae were conducted with pheromone traps and trap logs. We found more than 50 dead adults with symptoms white muscardine from several points under the bark. Healthy adults of Ips typographus without any pathogenic symptoms collected from pheromone traps and trap logs under bark in the forest Czech Bohemian Switzerland (?eske Svýcarsko). For adaptation bark beetles Ips typographus in the laboratory conditions and excluded any other abiotic factors as a potential reason for their mortality. They were placed in bark in the Petri plates with filter paper and treated by 1 ml of entomopathogenic fungi conidial suspensions.The isolates Bcz38 and Inc2 showed low level of mortality of Ips typographus . But the isolate Bcz2, Bcz8, Bcz9, Bcz12-Bcz25 of B . bassiana and Bcz31 B . pseudobassiana had high virulence against of Ips typographus 100%.
Highlights
One of the most dangerous groups of pest xylophages in coniferous forests is bark beetles (Coleoptera, Curculionidae, Scolytinae)
The monitoring of flight activity and development Ips typographus was studied in the several points of Czech Bohemian Switzerland (České Švýcarsko) with pheromone traps and logs, at an altitude of 340-471 m above sea level (N 50.927371°W 14.447837°; N 50.813300°W 14.388281°; N 50.866989°W14.319150°) in 2016 (Fig. 1)
In the process of isolating a pure culture of new isolates entomopathogenic fungi, they was identified respectively to morphological characteristics when growing on the medium Potato Dextrose Agar (PDA) and Sabouraud Dextrose Agar (SDA): Size, shape, structure, edge of colonies (Lednev et al, 2002)
Summary
One of the most dangerous groups of pest xylophages in coniferous forests is bark beetles (Coleoptera, Curculionidae, Scolytinae). The spruce bark beetle, Ips typographus (L.) (Coleoptera: Curculionidae, Scolytinae), is one of the most destructive insects infesting spruce forests in Europe (Faccoli and Bernardinelli, 2014). The outbreaks of Ips typographus (L.) in Central Europe after severe storms in the 1990s triggered extensive research (Wermelinger, 2004). The felling and removal of infested trees from forests are one of the most effective control methods (Wegensteiner and Weiser, 1996). There is a long-termdiscussion about possibility of sanitary cutting of trees infested by bark beetles in strictly protected areas. Protected zones of natural parks become often the areas with large-scale bark beetles outbreaks. No chemical treatment is used within the national park (KníÏek and Trýzna, 2014)
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