Abstract
Helminthosis are relevant because they are responsible by substantial economic losses. Many techniques have been developed to facilitate parasitological diagnosis and comparisons among these techniques are essential. The present work aimed a comparison among three techniques of quantitative sedimentation for parasitological diagnosis of Fasciola hepatica in cattle. The modified sedimentation techniques of Dennis, Stone & Swanson (DSS), Girão and Ueno (quatro tamises - QT; four sieves) and Foreyt were compared using analysis of variance, linear regression and correlation tests. Evaluations of sensitivity, specificity and agreement were performed using the kappa test. It was found that the modified DSS technique had a high mean egg count and high sensitivity (p<0.01) (48.60%). The three techniques showed a specificity of 100%. There was no correlation among the values of egg count obtained by the three techniques, and significant coefficients were not observed by regression analysis. The egg count results of F. hepatica obtained by the techniques of quatro tamises and Foreyt showed excellent concordance by the kappa test. The modified DSS technique appeared to be the most effective for F. hepatica diagnosis in cattle (p<0.01).
Highlights
Fasciolosis caused by the trematode Fasciola hepatica is a common zoonotic disease that affects cattle and sheep in endemic areas (Echevarria 2004; Faria et al 2005) and is considered as an emerging disease by the World Health Organization (MasComa et al 1999)
According to Cringoli et al (2002); Faria et al (2005), the economic losses caused by F. hepatica in cattle and sheep production are meaningful
Previous studies of prevalence indicate an increase in dispersion and incidence of this agent in endemic areas (Mas-Coma et al, 1999; Echevarria 2004; Dorchies 2007; Lima et al 2009)
Summary
Fasciolosis caused by the trematode Fasciola hepatica is a common zoonotic disease that affects cattle and sheep in endemic areas (Echevarria 2004; Faria et al 2005) and is considered as an emerging disease by the World Health Organization (MasComa et al 1999). According to Cringoli et al (2002); Faria et al (2005), the economic losses caused by F. hepatica in cattle and sheep production are meaningful. Previous studies of prevalence indicate an increase in dispersion and incidence of this agent in endemic areas (Mas-Coma et al, 1999; Echevarria 2004; Dorchies 2007; Lima et al 2009). These events probably are due to the dispersion of the intermediate hosts (lymnaeids aquatic mollusks) of this agent in some regions (Coelho; Lima 2003). Attempts to improve on existing techniques or their implementation and studies to review them would be very valuable
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
