Abstract
Two relatively high concentration 4% and 6% the replaceable Linear polyacrylamide (LPA) gel was used as the sieving medium. An extensive series of experiments has been performed using DNA fragments ranging in size from 80 to 587 base pairs; the properties such as the mobilities, the width at half height, and the resolution were compared. The electric field was changed from 100V/cm to 375V/cm. From the experiment results, it is shown: increasing the gel concentration from 4%LPA to 6%LPA can not improve the resolution to separate the DNA sample (the length of fragments600bp). This work complements our systematic study and will help to further optimize and improve high performance DNA separation in microfluidic applications.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
