Comparing the efficacy of EndoSequence Root Repair Material, Biodentine, MTA angelus as retrograde filling in microbial leakage by Confocal Laser Scanning Microscope

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This study aimed to compare the efficacy of EndoSequence ® Root Repair Materia, Biodentine, and MTA angelus® as root end filling in bacterial leakage and confirmed with Confocal Laser Scanning Microscope (CLSM). Methods: 55 maxillary single rooted teeth were used in this study. The teeth were decoronated at 16mm. root canals was prepared by using ProTaper rotary Ni-Ti instruments. Pro-taper F3 size Gutta-percha and AH plus sealer were used for single cone obturation technique. The root resection was done in the apex at 3 mm. The root end cavity was prepared by ultrasonic retro-tips. Samples were divided into 5 groups with 11 samples each according to retrograde cavities filling. Group1(EndoSequence), Group 2 (Biodentine). Group 3 (MTA angelus), Group 4 (Positive control), and Group 5 (Negative control). Dual chamber apparatus was prepared to test the retrograde filling leakage. The Enterococcus Faecalis in Confocal Laser Scanning Microscope under 20X magnification to measures of E. Faecalis penetration depth through retrograde materials. Results: EndoSequence® had better results in bacterial leakage and in penetration depth into the dentinal tubules with statisticallysignificant difference when compared to BiodentineTM and MTA angelus® (p ≤ 0.05). On other hand there was no any statistically significant difference between BiodentineTM and MTA angelus® (p>0.05) with more leakage in dual chamber whereas bacterial penetration into the dentinal tubules in CLSM was measured. Conclusions: EndoSequence showed better results in both bacterial leakage and in bacterial penetration.