Abstract
Objective: In the acid-fast staining experiment of pathological tissues, sulfuric acid and hydrochloric acid were used to evaluate the staining results, so as to get the best staining method. Methods: Using sulfuric acid differentiation solution and hydrochloric acid differentiation solution, the paraffin blocks of pathological tissues known to contain Mycobacterium tuberculosis were compared to evaluate the staining effect. Results: When 0.5% hydrochloric acid differentiation solution is used and the differentiation time is 6 s, the dyeing effect is better than that of sulfuric acid differentiation solution.
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