Abstract
Objective: To evaluate the antibacterial activity of gaseous ozone and chlorhexidine solution on a tooth cavity model. Study Design: Twenty-one human molars were divided into 3 groups. Cavities were then cut into the teeth (4 per tooth, 28 cavities per group). After sterilization, the teeth were left in broth cultures of 106 colony-forming units (CFU) ml-1 of Streptococcus mutans (S. mutans) at 36°C for 48 h. The appropriate treatment followed (group A, control; group B, 2% chlorhexidine solution; and group C, 80s of treatment with ozone, and the cavities were then filled with composite resin. After 72h, the restorations were removed, dentin chips were collected with an excavator, and the total number of microorganisms was determined. Results: Both of the treatments significantly reduced the number of S. mutans present compared with the control group and there was a significant difference between the all groups in terms of the amount of the microorganisms grown (p < 0.05). Group B was beter than group C; and group C was better than group A. Moreover, it was found that the amount of the growth in the group of chlorhexidine was significantly less than that of the ozone group (p < 0.05). Conclusion: Chlorhexidine solution was the antibacterial treatment most efficacious on S. mutans; however, ozone application could be an anlternative cavity disinfection method because of ozone’s cavity disinfection activity. Key words:Antibacterial activity, chlorhexidine, ozone, streptococcus mutans, tooth cavity.
Highlights
In 1993, Anderson et al [1] described the traditional treatment of dental caries as the surgical removal of the diseased parts of the tooth structure and obturation of the area with an inert filling material
The aim of the present study was to evaluate the antibacterial activity of gaseous ozone and chlorhexidine solution on a tooth cavity model
After the inoculated plates were incubated for 24 h, the number of the microorganisms that had grown on the plates was counted
Summary
In 1993, Anderson et al [1] described the traditional treatment of dental caries as the surgical removal of the diseased parts of the tooth structure and obturation of the area with an inert filling material. Ozone gas therapy has been suggested as an alternative noninvasive treatment aiming to reduce the levels of caries-associated microorganisms. The aim of the present study was to evaluate the antibacterial activity of gaseous ozone and chlorhexidine solution on a tooth cavity model. Each tooth was immersed in a bottle containing 5 ml of PYB medium, 50 μl of 106 CFU ml-1 S. mutans, and 1 % sucrose, incubated at 36 °C for 48 h in order to establish an infected cavity. Teeth with control cavities that were infected with S. mutans at a degree higher than 105 CFU g-1 were counted in the study. The composite fillings were removed using different sterile diamond burs, which were first placed in a freezer for cooling, without coming into contact with the dentin walls of the cavity. The level of significance was set at p < 0.05
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