Comparing the Accuracy and Efficiency of Leaf Gas Exchange Measurements of Excised and Nonexcised Strawberry (Fragaria ×ananassa Duch. ‘Camino Real’) Leaves

Abstract

Acquiring leaf gas exchange (LGE) data from field experiments is critical for numerous research endeavors. However, because of the extended time needed to perform measurements and the necessity for moving equipment, the number of leaf samples collected is often limited. To address this concern, two studies were conducted to evaluate the accuracy and efficiency of LGE measurements of excised and nonexcised strawberry leaves. Research was conducted in experimental field plots located in Lubbock, TX, USA, where ‘Camino Real’ strawberry leaves was sampled for LGE measurements, during the 2021 growing season. Using an auto program mode, two LI-6400/XT portable machines simultaneously measured the LGE of nearby selected leaves from the same plant. Measurements were recorded every 30 seconds. After 90 seconds, the petiole of one leaf was cut (excised leaf treatment), and the auto program continued for an additional 480 seconds. The results indicated that although the LGE for nonexcised leaves remained stable, excised leaf LGE changed after excision. Within 30 seconds, the postexcision stomatal conductance to water vapor (gsw) and net leaf photosynthetic rate (A) exhibited nearly 97% and 99% accuracy, respectively, of nonexcised leaf gsw and A. However, the excised LGE decrease accelerated over time, with gsw continuing to decrease by more than 5% after 43 seconds, indicating ≤95% accuracy of the gsw results. The data suggested that strawberry LGE may be measured accurately within 30 to 40 seconds after leaf excision. During a separate experiment, the mean time to complete each individual strawberry LGE measurement was 68.4 seconds (±0.9 seconds) for nonexcised leaves. Conversely, the mean time to complete each LGE measurement of excised leaves was 42.2 seconds (±0.2 seconds). Therefore, leaf excision appears to be a viable method of maintaining accuracy and increasing the sample size while collecting strawberry LGE data.