Comparing low-coherence interferometry and A-scan ultrasonography in measuring ocular axial dimensions in young rhesus monkeys

Abstract

We investigated a commercial low-coherence interferometer (LenStar LS 900 optical biometer) in measuring young rhesus monkey ocular dimensions. Ocular biometry data obtained using a LenStar and an A-scan ultrasound instrument (OPT-scan 1000) from 163 rhesus monkeys during 20–348 days of age were compared by means of coefficients of concordance and 95% limits of agreement. Linear regression was employed to examine and analyze the inter-instrument discrepancies. In young rhesus monkeys, the test-retest reliability of the LenStar was equal to or exceeded that of A-scan ultrasound (intraclass correlation = 0.86 to 0.93). The inter-instrument agreement was strong for vitreous chamber depth and axial length (coefficient of concordance = 0.95 and 0.86, respectively) and moderate for anterior chamber depth and lens thickness (coefficient of concordance = 0.74 and 0.63, respectively). The LenStar systematically underestimated ocular dimensions when compared to A-scan ultrasound (mean magnitude of difference = 0.11–0.57 mm). This difference could be minimized using linear calibration functions to equate LenStar data with ultrasound data. When this method was applied, the values between instruments were in excellent absolute agreement (mean magnitude of difference = 0.004–0.01 mm). In conclusion, the LenStar reliably measured ocular dimensions in young monkeys. When an appropriate calibration function is applied, the LenStar can be used as a substitute for A-scan ultrasonography.