Comparing BRIN-BD11 culture producing insulin using different type of microcarriers

Abstract

This research was conducted to examine the growth profile, growth kinetics, and insulin-secretory responsiveness of BRIN-BD11 cells grown in optimized medium on different types of microcarriers (MCs). Comparisons were made on modified polystyrene (Hillex(®) II) and crosslinked polystyrene Plastic Plus (PP) from Solohill Engineering. The cell line producing insulin was cultured in a 25cm(2) T-flask as control while MCs based culture was implemented in a stirred tank bioreactor with 1L working volume. For each culture type, the viable cell number, glucose, lactate, glutamate, and insulin concentrations were measured and compared. Maximum viable cell number was obtained at 1.47×10(5) cell/mL for PP microcarrier (PPMCs) culture, 1.35×10(5) cell/mL Hillex(®) II (HIIMCs) culture and 0.95×10(5) cell/mL for T-flask culture, respectively. The highest insulin concentration has been produced in PPMCs culture (5.31mg/L) compared to HIIMCs culture (2.01mg/L) and T-flask culture (1.99mg/L). Therefore overall observation suggested that PPMCs was likely preferred to be used for BRIN-BD11 cell culture as compared with Hillex(®) II MCs.