Compared effects of pinacidil, nicorandil and nitroglycerin on potassium channels in hypoxic and hypercapnic pulmonary vasoconstriction

Abstract

1.1. Possible contribution of K+ channel opening to the relaxation by glyceryl trinitrate (GTN) was examined using isolated rabbit aorta.2.2. While glibenclamide and apamin failed to affect relaxation by GTN, both charybdotoxin (ChTx) and iberiotoxin (IbTx) effectively attenuated GTN-induced relaxation.3.3. The increase in cGMP produced by GTN was not attenuated by ChTx and IbTx.4.4. The inhibitory effect of ChTx on GTN-induced relaxation was not reduced in the presence of zaprinast, indicating that cGMP but not GMP was responsible for activation of the K+ channel.5.5. Okadaic acid, a selective inhibitor of protein phosphatase 2A, had no effect on the relaxation by GTN. These results indicate that, though small in degree, activation of a ChTx-sensitive K+ channel (large conductance Ca2+-activated K+ channel) is involved in the GTN-induced relaxation in rabbit aorta.