Abstract

Chlorophyll is the green pigment in plants that helps to absorb sunlight and converts it into energy. It is believed that it is beneficial for the human body. Therefore in this study, the chlorophyll amount in three brown algae species (<i>Sirophysalis trinodis, Polycladia myrica</i> and <i>Colpomenia sinuosa</i>) of the Persian Gulf by using three solvents and applying two formulas was compared. Acetone, diethyl ether and methanol were used as solvents. Results showed that the chlorophyll amount in different species dependent on solvent type and cell wall structure of algae. The highest chlorophyll a and x+c contents were observed in <i>S. trinodis</i> species. Also, the highest content of chlorophyll c<sub>1</sub>+c<sub>2</sub> was showed in <i>C. sinuosa</i> by the extraction with diethyl ether solvent. In both formulas was no significant difference between the same solvents in the amount of chlorophyll a. In general, the aim of this study due to the diversity and dispersion of algae and also their potential use in the food industry, cosmetics and pharmaceutical goods was to choose a suitable solvent for better extraction of chlorophyll as an active ingredient. Hence, in this study, the acetone was a better solvent.

Highlights

  • Chlorophyll is a light-sensitive pigment with special electrical properties

  • The highest content of chlorophylls c1+c2 was observed in C. sinuosa by the extraction with diethyl ether solvent

  • Results indicated that there was a significant relationship between solvents used in the chlorophyll extraction (Table 2), especially between the acetone and others

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Summary

Introduction

Chlorophyll is a light-sensitive pigment with special electrical properties. All photosynthetic plants are containing chlorophyll a. The existence of subsidiary chlorophylls such as b, c and d is depending on the type of plant. Higher plants have two chlorophyll a and b, which chlorophyll b has a structure similar to chlorophyll a. The difference between these two chlorophylls is in their R groups (if R is a CH3 group, chlorophyll is the type of “a” and if R is a CHO group, chlorophyll is the type of “b”). The brown alga is lacking chlorophyll b [8]. The carotene level is depending on the algae species and environmental conditions [2]

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