Abstract
Objective In order to find out the most convenient,economic,accurate pretreatment methods for early endothelial progenitor cells (EPCs) detection from peripheral blood,this article compared the detection result of density gradient centrifugation method and two kinds of red cells lysis methods.Methods Arterial blood of New Zealand rabbits stored in vacuum heparin containing tubes was pretreated by 3 kinds of method respectively.The first pretreated method used lymphocyte separation liquid density gradient centrifugation,the second method used flow cytometry cell hemolysin and the third method used red cells lysis solution.After that,Anti-Mouse CD3-allophycocyanin (APC),Anti-Mouse CD45-phycoerythrin (PE),Anti-Mouse CD133 (Prominin-1)-fluorescein isothiocyanate (FITC) were labeled to detect early EPCs.Results There was no significant different of percentage of early endothelial progenitor cells pretreated by 3 kinds of method (P ＞ 0.05).Of the three methods,flow cytometry cell hemolysin method was the most convenient method for using the least blood,processing liquid,vitro and Instrument,not easy to be effected and repeating easily.Conclusion Flow cytometry cell hemolysin method was the most convenient pretreatment method for test endothelial progenitor cells percentage from peripheral blood. Key words: Pretreatment ; Early endothelial progenitor cells ; Lysis ; Density gradient centrifugation
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