Abstract
The present study aims to investigate the roles of herb pairs containing Angelicae Sinensis Radix (Danggui) in Xin-Sheng-Hua Granule (XSHG) on hemolytic and aplastic anemia (HAA) mice. HAA model mice were induced by acetyl phenylhydrazine and cyclophosphamide; then the samples of XSHG and its decomposed recipes (DY, DC, DT, DH, DJ, and DZ) were orally administrated to these mice. Indicators of peripheral blood routine, organ index, and ATPase activities were tested. Moreover, the main effective components in these samples were also analyzed by UHPLC-TQ-MS/MS. Clear separation between the control and model groups from score plot of principal component analysis (PCA) was easily seen, indicating that HAA model was successfully conducted. Afterwards, relative distance calculation method between dose groups and control group from PCA score plot was adopted to evaluate the integrated effects of hematinic function of different samples. And the orders of hematinic effects were as follows: XHSG > DJ > DT > DZ > DH > DC > DY. Further analysis of these samples by UHPLC-TQ-MS/MS revealed that XSHG underwent complicated changes when herb pairs containing Danggui were excluded from XSHG, respectively. Compared with XSHG, the vast majority of active compounds in sample DY (formula minus herb pair Danggui-Yimucao) decreased significantly, which could partly explain why herb pair Danggui-Yimucao made great contribution to XSHG. These findings showed that withdrawal analysis method is a valuable tool to analyze the impacts of herb pairs containing Danggui on XSHG, which could lay foundation to reveal the compatibility rules of this formula.
Highlights
Traditional Chinese medicines (TCMs) have made tremendous contributions to the health of the ancient Chinese people for a long time
Chemicals and Reagents. e herbs were generously provided by Revolence Pharmaceutical Co., Ltd. (Jiangsu, China), in February 2016, including Angelica sinensis (Oliv.) Diels, Leonurus japonicus Houtt., Ligusticum chuanxiong Hort., Prunus persica (L.) Batsch, Carthamus tinctorius L., roasted rhizome of Zingiber officinale Rosc, and Radix Glycyrrhizae Preparata; detailed information is summarized in Table S1. e seven herbs were kindly identified by Dr Qinan Wu, doctor of pharmacognosy (College of pharmacy, Nanjing University of Chinese Medicine, Nanjing, China), and the voucher specimens were deposited at the Herbarium of Nanjing University of Chinese Medicine
TCM formulae were the indispensable part of human life, which play significant roles for disease treatment in the developing countries
Summary
Traditional Chinese medicines (TCMs) have made tremendous contributions to the health of the ancient Chinese people for a long time. To achieve maximum therapeutic effect of certain diseases, TCMs are usually used in combination with several specific herbs instead of single herbs [1]. Xin-Sheng-Hua Granule (XSHG), originating from the Evidence-Based Complementary and Alternative Medicine classic and typical formulae Sheng-Hua Decoction, has function of promoting blood circulation, nourishing blood, and relieving pains [2]. According to the basic theory of TCM, XSHG is usually clinically applied to treat postpartum diseases including uterine hemorrhage, postpartum abdominal pain, and retention of the lochia after delivery [4]. It is well known that postpartum diseases are usually associated with blood deficiency. Erefore, studying the effects of nourishing blood of XSHG is significant for researchers to discover its action mechanism It is well known that postpartum diseases are usually associated with blood deficiency. erefore, studying the effects of nourishing blood of XSHG is significant for researchers to discover its action mechanism
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