Comparative transcriptomic analysis provides novel insights into the difference in textural alteration between mealy and crisp apple patterns

Abstract

Mealiness is a common textural deterioration of several fruit after harvest. To unravel the underlying mechanism involved in mealiness, biochemical characterization and global transcriptomic profiling were comparatively performed between mealy ‘Hongjiangjun’ (HJJ) and crisp ‘Fuji’ apples. Sensory evaluation and SEM-based microstructure observation showed that HJJ apples appeared to be mealy in only 3 d at 23 ± 1 °C, while Fuji apples did not appear to be mealy even after 28 d of storage. Textural deterioration and ethylene burst occurred more sharply in HJJ apples than in Fuji apples during storage. The results obtained from the dimensional RNA-sequencing analysis showed that a much stronger upregulation of the transcription of genes encoding polygalacturonase (PG), pectin acetylesterase (PAE), pectinesterase (PE), β-galactosidase (GAL), α-l-arabinofuranase (AF), and expansin (EXP) was observed in the pair of mealy HJJ apples vs. harvest than in the pair of Fuji apples after 28 d vs. harvest. The gene expression of ethylene responsive factor (ERF) was found to be strongly upregulated in HJJ apples compared with Fuji apples, which may mediate the regulation of downstream genes encoding cell wall-modifying enzymes. Weighted gene co-expression network analysis showed that the transcription factors MdbHLH63 and MdERF-like, and a constructure gene of MdGAL had strong connectivity with mealiness. Validation by qRT-PCR further confirmed the main findings obtained by RNA-sequencing. The occurrence of apple mealiness involves altered expression patterns of cell wall-modifying enzymes as well as MdbHLH63 and MdERF-like, which are core genes regulating the mealiness process. The above findings provide global insight into the difference in textural alteration between mealy and crisp apple patterns.