Comparative transcriptome combined with physiological analyses revealed key genes and pathways for cadmium tolerance in wild-type and mutant microalgae Dunaliella salina

Abstract

Cadmium (Cd) has become one of the major contaminants in aquatic ecosystems. The microalgae Dunaliella salina is known as a cadmium (Cd) accumulator and shows a high level of tolerance to Cd, but improved Cd tolerance and relevant mechanisms are still required for investigation. In this study, two mutant strains (M1 and M2) of D. salina with high Cd resistance were obtained by chemical mutagenesis. In the normal medium, there were no differences between the two mutant strains and the wild-type strain in biochemical and physiological indicators. However, the two mutant strains showed high Cd resistance, rapid growth rate, and good Cd accumulation capacity in the medium containing high levels of Cd compared with the wild strain, which may be involved in increases in protein content, accumulation of carotenoids and chlorophylls, and glutathione reductase (GR) activity. We identified 829, 587, and 740 differentially expressed genes (DEGs) from the Cd/Control, Cd + M1/Cd, and Cd + M2/Cd sample groups by transcriptome analysis, respectively. The main DEGs were found in the ribosome, DNA replication, photosynthesis-antenna, and photosynthesis pathways after KEGG enrichment. There were 39 DEGs involved in the photosynthesis and photosynthesis-antenna pathways, of which 28 DEGs were down-regulated by Cd in the wild-type strain but remained relatively unchanged in the mutant strains. Under Cd exposure, the mutant strains showed higher mRNA levels of most DEGs involved in the ribosome and DNA replication pathways than the wild strain. Taken together, the present study will help us understand the mechanism of Cd tolerance in microalgae, and provide candidate genes and key pathways for phytoremediation.