Abstract
BackgroundLight quality severely affects biosynthesis and metabolism-associated process of glutathione. However, the role of specific light is still unclear on the glutathione metabolism. In this article, comparatively transcriptome and metabolome methods are used to fully understand the blue and red-light conditions working on the glutathione metabolism in maize seedling leaf.ResultsThere are 20 differently expressed genes and 4 differently expressed metabolites in KEGG pathway of glutathione metabolism. Among them, 12 genes belong to the glutathione S-transferase family, 3 genes belong to the ascorbate peroxidase gene family and 2 genes belong to the ribonucleoside-diphosphate reductase gene family. Three genes, G6PD, SPDS1, and GPX1 belong to the gene family of glucose 6-phosphate dehydrogenase, spermidine synthase, and glutathione peroxidase, respectively. Four differently expressed metabolites are identified. Three of them, Glutathione disulfide, Glutathione, and l-γ-Glutamyl-L-amino acid are decreased while L-Glutamate is increased. In addition, Through PPI analysis, two annotated genes gst16 and DAAT, and 3 unidentified genes 100381533, pco105094 and umc2770, identified as RPP13-like3, BCAT-like1and GMPS, were obtained. By the analysis of protein sequence and PPI network, we predict that pco105094 and umc2770 were involved in the GSSG-GSH and AsA-GSH cycle in the network of glutathione metabolism.ConclusionsCompared to red light, blue light remarkably changed the transcription signal transduction and metabolism of glutathione metabolism. Differently expressed genes and metabolic mapped to the glutathione metabolism signaling pathways. In total, we obtained three unidentified genes, and two of them were predicted in current glutathione metabolism network. This result will contribute to the research of glutathione metabolism of maize.
Highlights
Light quality severely affects biosynthesis and metabolism-associated process of glutathione
Activity of enzyme associated with glutathione metabolism primary differ in the GSSG-GSH cycle
Expressed glutathione reductase (GR) and glutathione peroxidase (GPX) induced by blue light which regulated GSSG‐GSH cycle in glutathione metabolism Glutathione reductase (GR) is a key nicotinamide ademine dinucleotidephosphate (NADPH)-dependent flavo-protein oxidoreductase which can catalyze the glutathione disulfide (GSSG) to reduced glutathione (GSH) and protect plant cells from oxidative damage induced by reactive oxygen species (ROS) burst [18]
Summary
Light quality severely affects biosynthesis and metabolism-associated process of glutathione. The role of specific light is still unclear on the glutathione metabolism. Glutathione molecule is characterized by active sulfhydryl group, which is the most important functional group. Glutathione protects cell, preserves enzymes activities and proteins functions, prevents cytoplasmic and outer membranes damage [2]. These reactions prevent oxidative damage through the reduction of peroxides. Glutathione participates in carbohydrate metabolism, and can activate many enzymes, such as 6-phosphogluconate dehydrogenase, promoting the metabolism of carbohydrate, and protein [4]. Glutathione participates in the formation and maintenance of disulfide bonds in proteins and the transport of amino acids across cell membranes [5]
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