Comparative transcriptome analysis of Sogatella furcifera (Horv\xe1th) exposed to different insecticides

Cao Zhou,Gui-Yun Long,Dao-Chao Jin,Zhao Wang,Hong Yang

doi:10.1038/s41598-018-27062-4

Cao Zhou, Gui-Yun Long + Show 3 more

Open Access

https://doi.org/10.1038/s41598-018-27062-4

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Abstract

White-backed planthopper, Sogatella furcifera (Horváth) (Hemiptera: Delphacidae), one of the main agricultural insect pests in China, is resistant to a wide variety of insecticides. We used transcriptome analysis to compare the expression patterns of resistance- and stress-response genes in S. furcifera subjected to imidacloprid, deltamethrin, and triazophos stress, to determine the molecular mechanisms of resistance to these insecticides. A comparative analysis of gene expression under imidacloprid, deltamethrin, and triazophos stress revealed 1,123, 841, and 316 upregulated unigenes, respectively, compared to the control. These upregulated genes included seven P450s (two CYP2 clade, three CYP3 clade, and two CYP4 clade), one GST, one ABC transporter (ABCF), and seven Hsps (one 90 and six Hsp70s) under imidacloprid stress; one P450 (CYP3 clade), two ABC transporters (one ABCF and one ABCD), and one Hsp (Hsp90) under deltamethrin stress; one P450 (CYP3 clade) and one ABC transporter (ABCF) under triazophos stress. In addition, 80 genes were commonly upregulated in response to the three insecticide treatments, including laminin, larval cuticle protein, and fasciclin, which are associated with epidermal formation. These results provide a valuable resource for the molecular characterisation of insecticide action in S. furcifera, especially the molecular characteristics of insecticide cross resistance.

Highlights

The white-backed planthopper (WBPH), Sogatella furcifera (Horváth) (Hemiptera: Delphacidae) is a major pest in rice paddies throughout Asia
Quantitative PCR revealed that eight ATP-binding cassette (ABC) transporters from the ABCB/C/D/G subfamilies were overexpressed in related strains of Laodelphax striatellus resistant to chlorpyrifos, deltamethrin, and imidacloprid, compared to in the susceptible strain[14]
We aimed to identify a wide range of genes, including those encoding detoxification enzymes induced by insecticides used for the control of S. furcifera, and at the same time lay a foundation for understanding the resistance mechanisms of pests to different types of insecticides

Summary

Introduction

The white-backed planthopper (WBPH), Sogatella furcifera (Horváth) (Hemiptera: Delphacidae) is a major pest in rice paddies throughout Asia. Rice planthopper resistance has been reported following the long-term and widespread spraying of imidacloprid in the field[5], and it was suggested that its use to control N. lugens in China was ceased. We used the Illumina HiSeq2000 (Illumina, San Diego, CA, USA) to produce a de novo transcriptome, and the BGIseq-500 (BGI, Wuhan, China; http://www.seq500.com/en/) platform to quantify expression levels of S. furcifera genes as a resource to provide insights into insecticide-related changes in gene expression following treatment with imidacloprid, triazophos, and deltamethrin. We aimed to identify a wide range of genes, including those encoding detoxification enzymes induced by insecticides used for the control of S. furcifera, and at the same time lay a foundation for understanding the resistance mechanisms of pests to different types of insecticides

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