Abstract
We studied the molecular mechanism of the quality traits of wood formation in larch. We used the immature latewood cells of two Japanese larch (Larix kaempferi) clones with significant differences in density and in microfibrillar angle (MFA) as materials to analyze their gene expression profiles. A total of 1735 differentially expressed genes were detected in immature latewood cells of the two clones, among which, 971 were up-regulated and 764 were down-regulated. Digital gene expression profiling analysis revealed that genes encoding transcription factor members NAC66 and R2R3-MYB4, microtubule-associated protein, actin-related protein, cell wall protein members, arabinogalactan protein, Fasciclin-like arabinogalactan protein and glycine-rich protein, and several cell-wall-synthesis genes affected wood density and MFA by regulating latewood formation at transcriptional level. Our study results represent a basis for selection of quality traits and genetic improvement of larch wood.
Highlights
Forest breeders aim to genetically improve the mechanical properties of wood and fiber by selecting traits that can yield high-quality wood products
We studied the molecular mechanism of the quality traits of wood formation in larch
We studied two Japanese larch (Larix kaempferi) clones with significant differences in wood density and microfibrillar angle (MFA) to analyze the gene expression patterns of immature latewood cells at transcription level by using digital gene expression profiling (DGE)
Summary
Forest breeders aim to genetically improve the mechanical properties of wood and fiber by selecting traits that can yield high-quality wood products. Wood density reflects the accumulation of cell wall components while microfibrillar angle (MFA) reflects the structure of the fiber in the cell wall (Evans and Ilic 2001). These two traits influence the mechanical properties of wood. Wood property traits are complex and are regulated through the expression of multiple genes at the transcription level (Hertzberg et al 2001; Schrader et al 2004). Analysis of wood-formation transcription regulation is the basis for breeding improvement through selection of quality traits. Researchers have increasingly focused on isolation and identification of wood-related genes associated with transcription regulation and quality traits by using genetic engineering and gene localization and transcription techniques
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