Abstract
BackgroundThe renal function plays a leading role in long-term control of arterial pressure. The comparative analysis of renal cortex transcriptome in ISIAH rats with inherited stress-induced arterial hypertension and normotensive WAG rats was performed using RNA-Seq approach. The goal of the study was to identify the differentially expressed genes (DEGs) related to hypertension and to detect the pathways contributing to the differences in renal functions in ISIAH and WAG rats.ResultsThe analysis revealed 716 genes differentially expressed in renal cortex of ISIAH and WAG rats, 42 of them were associated with arterial hypertension and regulation of blood pressure (BP). Several Gene Ontology (GO) terms significantly enriched with DEGs suggested the existence of the hormone dependent interstrain differences in renal cortex function. Multiple DEGs were associated with regulation of blood pressure and blood circulation, with the response to stress (including oxidative stress, hypoxia, and fluid shear stress) and its regulation. Several other processes which may contribute to hypertension development in ISIAH rats were: ion transport, regulation of calcium ion transport, homeostatic process, tissue remodeling, immune system process and regulation of immune response.KEGG analysis marked out several pathways significantly enriched with DEGs related to immune system function, to steroid hormone biosynthesis, tryptophan, glutathione, nitrogen, and drug metabolism.ConclusionsThe results of the study provide a basis for identification of potential biomarkers of stress-sensitive hypertension and for further investigation of the mechanisms that affect renal cortex function and hypertension development.Electronic supplementary materialThe online version of this article (doi:10.1186/s12863-015-0306-9) contains supplementary material, which is available to authorized users.
Highlights
The renal function plays a leading role in long-term control of arterial pressure
The analysis revealed 716 genes differentially expressed in the renal cortex of hypertensive Inherited Stress-Induced Arterial Hypertension (ISIAH) and normotensive Wistar Albino Glaxo (WAG) rats
The expression of nine of these genes was detected in renal cortex of WAG rats but not in ISIAH
Summary
The renal function plays a leading role in long-term control of arterial pressure. The goal of the study was to identify the differentially expressed genes (DEGs) related to hypertension and to detect the pathways contributing to the differences in renal functions in ISIAH and WAG rats. Many researchers agree that the renal function plays a leading role in long-term control of arterial pressure. The use of experimental animal models provides valuable information to elucidate the nature of polygenic traits [4]. One of these is the ISIAH (Inherited Stress-Induced Arterial Hypertension) rat strain which was developed to study the mechanisms of the stress-sensitive hypertension and its complications.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
