Comparative toxicity of green and chemically synthesized CuO NPs during pregnancy and lactation in rats and offspring: Part I -hepatotoxicity

Abstract

Widespread applications of nanomaterials in food and health sciences have inevitable toxicological outcomes. Among nanomaterials, copper oxide nanoparticles (CuO NPs) are commonly used in all fields due to its distinctive characteristics. The study was designed to investigate the comparative hepatotoxic effect of green (GNPs) and chemically synthesized (CNPs) CuO NPs on spargue-dawely rats and their offspring. NPs (50 and 100 mg/kg) were orally administered to rats twice a week starting before mating. After birth the parents were continued dosing while pups were only on mother feed. Antioxidant, lipid peroxidation, genotoxicity, and histology were performed on liver tissue. In addition serum biochemistry of parents and offspring was also performed. The levels of catalase, peroxidase, and glutathione were significantly lowered only in CNPs treated parents while lipid peroxidation level was increased in these groups. Maximum genotoxicity (2.3%) in terms of percent tail DNA was observed in parent rats administered with high CNPs dose while other groups did not exhibit significant variation in genetoxic parameters. Gender and dose dependent effects were observed on liver function tests especially ALP and ALT in parents however no obvious differences were observed in offspring. Furthermore, dose dependent dilation and congestion of sinusoids was observed on CNPs administration. In GNPs treated rats and offspring histological alterations were observed. The study concludes that chemically synthesized CuO NPs exhibit dose dependent toxic effects on liver as compared to green synthesized CuO NPs. Furthermore lactation does not play significant role in the hepatotoxicity of offspring though minor oxidative stress was observed only on CNPs administration. The study also shows that pharmacological application of green synthesized NPs can be accomplished due to their biocompatible nature.