Abstract
A simple TLC and precise RP-HPLC method was developed and validated to standardize the three ambiguous botanical sources of Mayurasikha with the help of two active markers (stigmasterol and oleanolic acid) compounds. TLC study of the three plant extracts showed spots with different Rf Values. In HPLC analysis, separation was through a Phenomenex Luna C18 column with mobile phase consisting of acetonitrile: water 87:13 (v/v) at 210 nm. The retention times of stigmasterol and oleanolic acid were about 2.36 and 7.53 min resp. and the developed method shown good sensitivity, significant linearity (r2 ≥0.9992) over the concentration ranges 2 ̶12 and 50 ̶ 500 µg/ml. The mean percentage recovery of stigmasterol was found to be 97.46 ̶100.31% and for oleanolic acid 99.32 ̶ 99.64%. Hence, the combination of TLC and RP-HPLC fingerprinting and quantitative analysis can be handy in identification and quality evaluation of the three ambiguous sources of Mayurasikha.
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