Abstract
This study compared nested polymerase chain reaction (PCR) methods with the traditional Duncan bait test for the detection of Phytophthora fragariae var. fragariae in 86 root samples representing 30 strawberry fruit crops in England and Wales. DNA was extracted directly from the sampled roots using a cetyltrimethyl ammonium bromide‐based (CTAB) extraction method. Dilutions of the extracted DNA were then amplified using a nested PCR protocol. The PCR results correlated strongly with those of the bait tests, with the PCR methodology appearing to be the more reliable detection method on this type of field material. The PCR test also had the advantage of being a more rapid, less labour and time‐intensive test, compared with the 5‐6 week bait test. Comparisons were also made with a second set of nested PCR primers on DNA extracted from infected roots from the same field material; both primer sets performed equally well.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.